Cholestasis: Lamellar Structure of the Abnormal Human Serum Lipoprotein

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Science  30 Apr 1971:
Vol. 172, Issue 3982, pp. 475-478
DOI: 10.1126/science.172.3982.475


An abnormal lipoprotein was visualized directly in serum by electron microscopy of preparations negatively stained with potassium phosphotungstate. It appears as a unique disk-shaped particle with major axis measuring 400 to 600 angstroms and minor axis measuring about 100 angstroms. Chemical analysis, viscosity measurements, and x-ray diffraction analysis of purified preparations indicate that the particle, consisting of a one-to-one molar mixture of cholesterol and choline phosphatides associated with a small amount of protein, is a flattened vesicle, the wall of which is a continuous lipid bilayer.

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