Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations

See allHide authors and affiliations

Science  22 Sep 1972:
Vol. 177, Issue 4054, pp. 1104-1105
DOI: 10.1126/science.177.4054.1104


Isolated rat brain tubulin can be repolymerized in vitro in solutions containing adenosine triphosphate or guanosine triphosphate, magnesium ions, and a good calcium chelator. The extreme sensitivity of tubulin to calcium ions explains the failure of previous efforts to obtain polymerization and suggests a possible mechanism for regulation of microtubule polymerization in vivo.

Stay Connected to Science