Reaction of Hydrated Electrons with Ferricytochrome c

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Science  16 Feb 1973:
Vol. 179, Issue 4074, pp. 680-682
DOI: 10.1126/science.179.4074.680


The reaction of ferricytochrome c with hydrated electrons produced at pH 6.8 by radiolysis with electron pulses lasting 50 to 1000 nanoseconds has a specific rate constant of 5.5 x 1010 liter mole-1 sec-1 for the formation of the primary adduct. By using appropriate wavelengths, another fast, consecutive process was demonstrated, with the pure first order rate constant 1.0 x 105 sec-1. Its characteristics agree with it being an intramolecular process within the enzyme. Approximately 50 percent of all electrons which add to ferricytochrome c end in forming ferrocytochrome c.