Mycoplasma Contamination of Cultured Amniotic Fluid Cells: Potential Hazard to Prenatal Chromosomal Diagnosis

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Science  26 Apr 1974:
Vol. 184, Issue 4135, pp. 477-480
DOI: 10.1126/science.184.4135.477


Amniotic fluid cell cultures were screened for mycoplasma contamination. Mycoplasma RNA's were observed in more than half the cultures examined. Karyotypic analyses of these contaminated cell cultures revealed a significant increase in chromosomal aberrations. These studies emphasize the need for screening for mycoplasma in cultured amniotic cells.