A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization

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Science  26 Mar 1976:
Vol. 191, Issue 4233, pp. 1266-1268
DOI: 10.1126/science.1083070


Cytoplasm of maturing amphibian oocytes possesses a factor that induces germinal vesicle breakdown. This factor was extracted from Rana pipiens eggs and assayed by microinjection in Xenopus laevis oocytes. The activity of this factor is Mg-dependent, Ca-sensitive, and associated with heat-labile protein. Centrifugation on a sucrose density gradient revealed that the factor exists in three different molecular sizes.

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