Antibodies to histones and histone-histone complexes: immunochemical evidence for secondary structure in histone 1

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Science  30 Apr 1976:
Vol. 192, Issue 4238, pp. 469-471
DOI: 10.1126/science.3853


Highly specific antibodies were raised to histone 1 (H 1) and the histone complexes H32-H42 AND H2A-H2B, isolated by salt extraction. Antibody to H1 could detect irreversible conformational changes in acid- or urea-treated H1. The antibodies showed different reactivities with chromosomes as compared to antibodies in acid-extracted histones and should be useful in studies of native chromatin and chromosome structure.