Lens culinaris lectin immobilized on sepharose: binding and sugar-specific release of intact tissue culture cells

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Science  30 Apr 1976:
Vol. 192, Issue 4238, pp. 487-489
DOI: 10.1126/science.1257785


Lens culinaris lectin (LCL) covalently linked to 2B Sepharose binds tissue culture cells to the matrix. This is prevented by hapten sugars specific for LCL. Unlike other immobilized lectins, lens culinaris lectin allows the removal of bound cells from the matrix on addition of the specific sugars in a concentration-dependent manner. Binding and release occur under physiological conditions. Released cells continue to grow.