Denitrification Studies with 13N-Labeled Nitrate

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Science  18 Jun 1976:
Vol. 192, Issue 4245, pp. 1229-1231
DOI: 10.1126/science.192.4245.1229


Nitrate labeled with 13N (13NO3) was produced in a cyclotron by the 16O(p, α)13N reaction with protons having energies of 14.5 million electron volts. The 13NO3 was used as a tracer for direct quantitative measurements of denitrification rates in soils from flooded rice fields. The 13N technique provides a new tracer method for the measurement of denitrification rates in natural systems over short time intervals, without changing the concentration of NO3in the system.