Reports

Human malaria parasites in continuous culture

See allHide authors and affiliations

Science  20 Aug 1976:
Vol. 193, Issue 4254, pp. 673-675
DOI: 10.1126/science.781840

Abstract

Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. The have remained infective to Aotus.

Stay Connected to Science