Multiplication of a human parasite (Leishmania donovani) in phagolysosomes of hamster macrophages in vitro

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Science  20 Aug 1976:
Vol. 193, Issue 4254, pp. 678-680
DOI: 10.1126/science.948742


Leishmania donovani, the etiological agent of human visceral leishmaniasis, was grown in hamster peritoneal macrophages in vitro. By electron microscopy, using a lysosomal marker, these parasitic protozoa were seen to multiply within host cell phagolysosomes. The survival mechanism of this intracellular parasite is based apparently upon resistance to macrophage lysosomal enzymic digestion.