Selective in vitro growth of T lymphocytes from normal human bone marrows

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Science  10 Sep 1976:
Vol. 193, Issue 4257, pp. 1007-1008
DOI: 10.1126/science.181845


Selective growth of T lymphocytes occurred when unfractionated normal human bone marrow cells were cultured with conditioned medium obtained from phytohemagglutinin-stimulated normal human lymphocytes (Ly-CM). Cultures of up to 90 percent T cells have been maintained for more than 9 months. The T cells exhibited a strict growth dependence upon Ly-CM and were consistently negative for Epstein-Barr viral information.

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