Phosphatases in lake water: characterization of enzymes from phytoplankton and zooplankton by gel filtration

See allHide authors and affiliations

Science  15 Oct 1976:
Vol. 194, Issue 4262, pp. 320-321
DOI: 10.1126/science.184531


Sephadex gel filtration was used to characterize phosphomonoesterases in two small takes in northern Sweden. Two fractions, here termed phosphatase A and phosphatase B, were found both as free enzymes and associated with seston. The activity of phosphatase A was correlated with the presence of algal biomass. Phosphatase B, on the other hand, was derived from zooplankton. Phosphate served as an effective inhibitor of phosphatase A but had no such effect on phosphatase B. Both fractions had pH optima between 6.5 and 7.0.