Kinetic resonance Raman spectroscopy: dynamics of deprotonation of the Schiff base of bacteriorhodopsin

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Science  25 Mar 1977:
Vol. 195, Issue 4284, pp. 1328-1330
DOI: 10.1126/science.841330


We have developed a kinetic technique, combining resonance Raman spectroscopy and variable-speed continuous flow methods, to study molecular dynamics of isolated sites in macromolecules. Kinetic resonance Raman spectra of the retinylidence chromophore of bacteriorhodopsin have been obtained and the dynamics of the deprotonation of the Schiff base linkage is discussed.

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