Construction of chimeric phages and plasmids containing the origin of replication of bacteriophage lambda

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Science  09 Dec 1977:
Vol. 198, Issue 4321, pp. 1041-1046
DOI: 10.1126/science.929185


Segments of the replication control region of bacteriophage lambda (lambda) and lambda mutants defective in replication were attached in vitro to the phi80 phage vector Charon 3 and to the plasmid vector mini Col El (pVH51). The chimeric phages and plasmids have been used to localize the origin of lambda DNA replication and to facilitate a structural analysis of the lambda replicator.

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