Physical structure of the replication origin of bacteriophage lambda

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Science  09 Dec 1977:
Vol. 198, Issue 4321, pp. 1051-1056
DOI: 10.1126/science.929187

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The nucleotide sequence of part of the replication region of wild-type bacteriophage lambda and of four mutants defective in the origin of DNA replication (ori-) has been determined. Three of the ori- mutations are small deletions, and one is a transversion. The sequence of the origin region, defined by these mutations, contains a number of unusual features.

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