Localization of the globin gene in the template active fraction of chromatin of Friend leukemia cells

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Science  16 Dec 1977:
Vol. 198, Issue 4322, pp. 1166-1168
DOI: 10.1126/science.270812


Friend leukemia cell chromatin has been fractionated into template active and inactive components. The globin gene sequence is associated with the template active component both prior to and after the cells are induced with dimethyl sulfoxide to synthesize hemoglobin and therefore appears to be in an active configuration in uninduced as well as in induced Friend leukemia cells. In cells which have lost the ability to produce hemoglobin, the globin gene sequence is not associated with the template active fraction of chromatin. These results demonstrate the success of the fractionation procedure.