Cholinergic Vesicles: Ability to Empty and Refill Independently of Cytoplasmic Acetylcholine

See allHide authors and affiliations

Science  06 Jan 1978:
Vol. 199, Issue 4324, pp. 85-86
DOI: 10.1126/science.199.4324.85


Incubation of minced mouse-forebrain tissues in lithium Krebs solution reduces the acetylcholine content of the vesicular fraction 70 percent without altering that of the cytoplasmic fraction. Depleted vesicular-bound acetylcholine can be restored with newly synthesized acetylcholine (formed from extracellular choline) independently of the cytoplasmic pool. Depletion of vesicular-bound acetylcholine does not facilitate the movement of preformed extracellular-acetylcholine into vesicles.