Switch in immunoglobulin class production observed in single clones of committed lymphocytes

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Science  10 Mar 1978:
Vol. 199, Issue 4333, pp. 1078-1080
DOI: 10.1126/science.305113


Mouse spleen cells, after stimulation with lipopolysaccharide, were cloned in culture. After 4 to 5 days, the daughter cells were stained and examined for immunoglobulin class with double immunofluorescent reagents. A switch of the stained color of these cells was observed, implying a switch from imunoglobulin M to immunoglobulin G production in the progeny of a single B cell.