Phosphorylation of Isocitrate dehydrogenase of Escherichia coli

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Science  16 Mar 1979:
Vol. 203, Issue 4385, pp. 1111-1112
DOI: 10.1126/science.34215


Addition of acetate to a stationary phase culture of Escherichia coli in glycerol mineral salts medium containing phosphorus-32-labeled orthophosphate results in rapid loss of isocitrate dehydrogenase activity and concomitant incorporation of phosphorus-32 into the enzyme. This is the first example of protein phosphorylation in a bacterium in which the endogenous substrate for the protein kinase has been identified.