Cell-Free Nitrogenase and Hydrogenase from Actinorhizal Root Nodules

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Science  17 Aug 1979:
Vol. 205, Issue 4407, pp. 688-689
DOI: 10.1126/science.205.4407.688


Field-grown alder (Alnus glutinosa) root nodules were disrupted in liquid nitrogen to release the actinomycete endophytes. The endophytes were broken by mild sonic oscillation and yielded a cell-free nitrogenase preparation capable of reducing acetylene and protons. In addition, the preparation carried a cell-free uptake hydrogenase.