Cloned Cauliflower Mosaic Virus DNA Infects Turnips (Brassica rapa)

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Science  13 Jun 1980:
Vol. 208, Issue 4449, pp. 1265-1267
DOI: 10.1126/science.208.4449.1265


Cauliflower mosaic virus DNA cloned in the Sal I site of bacterial plasmid pBR322 infects turnip plants. The cloned viral DNA must be excised from the recombinant plasmid to infect, but need not be circularized and ligated in vitro. The cloned viral DNA lacks site-specific single-strand breaks found in DNA obtained directly from the virus. However, these breaks are reintroduced into the viral genome during multiplication of the virus in the plant host.