Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites

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Science  12 Sep 1980:
Vol. 209, Issue 4462, pp. 1240-1242
DOI: 10.1126/science.7403880


A mouse macrophage line, J774G8, supports continuous and prolific intracellular growth of Leishmania mexicana amazonensis, the etiological agent of a South American cutaneous leishmaniasis. The intracellular parasites from these infected cultures can be isolated with high recovery rate and purity by simple Percoll gradient centrifugation.