Cell lineage analysis by intracellular injection of fluorescent tracers

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Science  26 Sep 1980:
Vol. 209, Issue 4464, pp. 1538-1541
DOI: 10.1126/science.6159680


Cell lineages during development of the leech are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. Use of this peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast.