Inhibition of biological activity of mouse beta-nerve growth factor by monoclonal antibody

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Science  21 Nov 1980:
Vol. 210, Issue 4472, pp. 910-912
DOI: 10.1126/science.6159686


A continuous hybrid cell line was derived that secretes monoclonal antibody capable of inhibiting the biological activity of mouse beta-nerve growth factor (beta-NGF). Results obtained with monovalent fragments indicate that the monoclonal antibody inhibits activity by interfering with the direct interaction between beta-NGF and the cell membrane receptor rather than by precipitating the dimeric form of beta-NGF. This monoclonal antibody binds to an antigenic determinant common to mouse beta-NGF, snake venom (Naja naja) beta-NGF, and human beta-NGF. These antibodies should provide specific molecular probes for a variety of studies of nerve growth factor including its tissue distribution and mechanism of action.