Nullisomic Tetrahymena: Eliminating Germinal Chromosomes

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Science  31 Jul 1981:
Vol. 213, Issue 4507, pp. 549-551
DOI: 10.1126/science.213.4507.549


Germinal and somatic functions in Tetrahymena are performed separately by the micro- and macronuclei, respectively. Cells with haploid micronuclei were mated with diploids to yield monosomic progeny. These were induced to undergo a form of self-fertilization, generating cells lacking both copies of one or more of the five chromosomes in the micronucleus while still possessing a complete macronuclear genome.