Computer averaging of electron micrographs of 40S ribosomal subunits

See allHide authors and affiliations

Science  18 Dec 1981:
Vol. 214, Issue 4527, pp. 1353-1355
DOI: 10.1126/science.7313694


An enhanced lateral view of the 40S ribosomal subunit of HeLa cells has been obtained by computer averaging of single particles visualized in the electron microscope. Application of crystallographic criteria to independent averages shows that the reproducibility of the result is comparable to that obtained for thin, stained protein crystals by conventional Fourier filtration methods.

Stay Connected to Science