Proliferative capacity of murine hematopoietic stem cells in vitro

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Science  26 Mar 1982:
Vol. 215, Issue 4540, pp. 1619-1622
DOI: 10.1126/science.7071580


Large numbers of granulocytes can be collected repeatedly from the supernatant medium of long-term cultures of mouse bone marrow cells. A constant relationship was found between the number of adherent hematopoietic stem cells and the lifetime cell production per culture. The data indicate that there is a limit to the proliferative capacity of normal and of irradiated stem cells. A similar limitation was found in the production of marked granulocytes from clonal cultures of "beige" C57 (bg/bgJ) stem cells placed in limiting dilutions into stromal culture layers.