Active genes are sensitive to deoxyribonuclease I during metaphase

See allHide authors and affiliations

Science  13 Aug 1982:
Vol. 217, Issue 4560, pp. 648-650
DOI: 10.1126/science.6283640


The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.

Stay Connected to Science