Neuronal mapping: a photooxidation reaction makes Lucifer yellow useful for electron microscopy

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Science  03 Sep 1982:
Vol. 217, Issue 4563, pp. 953-955
DOI: 10.1126/science.7112109


Irradiation Lucifer yellow-filled neurons with intense blue light in the presence of 3,3'-diaminobenzidine produces an electron-opaque osmiophilic polymer within the injected cells. This technique is valuable when cobalt or horseradish peroxidase injections are difficult or when a second intracellular marker is needed to demonstrate neuronal contacts.