Microholography of Living Organisms

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Science  15 Oct 1982:
Vol. 218, Issue 4569, pp. 229-235
DOI: 10.1126/science.218.4569.229


By using intense pulsed coherent x-ray sources that are currently under development, it will be possible to obtain magnified three-dimensional images of elementary biological structures in the living state at precisely defined instants. For optimum contrast, sensitivity, and resolution, the hologram should be made with x-rays tuned to a resonance of nitrogen near 0.3 nanometer. Resolution will then be limited mainly by the hydrodynamic expansion that occurs while the necessary number of photons is being registered. Problems of technique are also briefly discussed.

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