Bacterial bioluminescence: isolation and expression of the luciferase genes from Vibrio harveyi

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Science  19 Nov 1982:
Vol. 218, Issue 4574, pp. 791-793
DOI: 10.1126/science.10636771


Genes for the luciferase enzyme of Vibrio harveyi were isolated in Escherichia coli by a general method in which nonluminous, transposon insertion mutants were used. Conditions necessary for light production in E. coli were examined. Stimulation of transcription of the genes for luciferase (lux A and lux B) was required for efficient synethesis of luciferase. To enhance transcription bacteriophage promoter elements were coupled to the cloned lux gene fragments.

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