Recombination during gene transfer into mouse cells can restore the function of deleted genes

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Science  14 Jan 1983:
Vol. 219, Issue 4581, pp. 174-176
DOI: 10.1126/science.6294829


Two plasmids containing nonoverlapping deletions of the herpes simplex virus thymidine kinase gene were introduced into thymidine kinase-deficient mouse L cells by DNA-mediated gene transfer. Thymidine kinase-producing transformants were generated by a mixture of the two plasmids at a frequency significantly greater than that generated by either plasmid alone. Southern blot analyses demonstrated that functional thymidine kinase genes were generated by homologous recombination between the two deletion mutants.

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