Protein sequence analysis: automated microsequencing

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Science  11 Feb 1983:
Vol. 219, Issue 4585, pp. 650-659
DOI: 10.1126/science.6687410


The automated microsequencing of proteins can now be carried out at the 5- to 10-picomoles (submicrogram) level on polypeptides obtained directly from one- and two-dimensional gel electrophoresis. The techniques are applicable to polypeptides ranging in size from small peptides (less than 10 residues) to large proteins (more than 1000 residues).