Rapid Flow Cytometric Analysis of the Cell Cycle in Intact Plant Tissues

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Science  03 Jun 1983:
Vol. 220, Issue 4601, pp. 1049-1051
DOI: 10.1126/science.220.4601.1049


Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.

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