Expression of streptococcal M protein in Escherichia coli

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Science  19 Aug 1983:
Vol. 221, Issue 4612, pp. 758-760
DOI: 10.1126/science.6192499


The structural gene for group A streptococcal M protein, the fibrillar surface molecule enabling the organism to resist phagocytosis, has been cloned into Escherichia coli. The molecule produced by Escherichia coli is slightly larger than the M protein isolated by solubilization of the streptococcal cell wall, but is similar in size to that secreted by streptococcal protoplast and L forms. Immunologically, the molecule synthesized by Escherichia coli has the same type-specific determinants as the streptococcal M protein.

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