X chromosome-linked transmission and expression of retroviral genomes microinjected into mouse zygotes

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Science  19 Aug 1983:
Vol. 221, Issue 4612, pp. 760-762
DOI: 10.1126/science.6683871


A genomic clone consisting of the Moloney leukemia proviral genome with moderately repetitive mouse sequences was microinjected into the pronucleus of a mouse zygote. An animal was derived that carried multiple copies of proviral DNA in a tandem array. No evidence for homologous recombination was obtained. The viral genome was expressed in this animal and was transmitted as a single unit to its offspring. Subsequent breeding studies revealed that the proviral DNA had integrated on an X chromosome.