Detection of antibodies to herpes simplex virus with a continuous cell line expressing cloned glycoprotein D

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Science  04 Nov 1983:
Vol. 222, Issue 4623, pp. 524-527
DOI: 10.1126/science.6312563


The gene for glycoprotein D of herpes simplex virus type 1 (HSV-1) was expressed in stable mammalian cell lines. Glycoprotein D produced in these cells has a number of antigenic determinants in common with the native glycoprotein. Cell lines expressing glycoprotein D were used in an enzyme-linked immunosorbent assay to detect human antibodies to glycoprotein D. This strategy should prove useful in determining the extent to which the immune response to HSV-1 is directed toward glycoprotein D.