Functional expression of a cloned I-A beta k gene in B-lymphoma cells

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Science  24 Feb 1984:
Vol. 223, Issue 4638, pp. 825-828
DOI: 10.1126/science.6420890


The immune response genes of the mouse encode two cell-surface glycoproteins, I-A and I-E, that play critical roles in determining the animal's immune responsiveness. The I-A antigen contains two chains, alpha and beta. A cloned beta-chain gene, I-A beta k, was introduced into B-lymphoma cells that express I-Ad. The transfected gene was successfully expressed on the cell surface of the recipient cells and was functional in stimulating allospecific T cells.