Temperature Effects on the Rate of Ty Transposition

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Science  05 Oct 1984:
Vol. 226, Issue 4670, pp. 53-55
DOI: 10.1126/science.226.4670.53


An assay has been developed to measure the rate of transposition of the transposable element Ty in Saccharomyces cerevisiae. The assay is based on the altered expression of the glucose-repressible alcohol dehydrogenase gene of yeast upon insertion of a Ty in front of this gene. By this assay the transposition rate of Ty elements was found to increase approximately 100-fold at temperatures lower than 30°C, the optimum growth temperature for Saccharomyces cerevisiae.

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