Functional properties of individual neuronal branches isolated in situ by laser photoinactivation

See allHide authors and affiliations

Science  19 Apr 1985:
Vol. 228, Issue 4697, pp. 344-346
DOI: 10.1126/science.3983633


The functional properties of an isolated dendritic branch of an identified sensory interneuron in the cricket were studied. The branch responded to wind stimuli directed at the animal and displayed a distinct directional sensitivity to those stimuli. A technique was used that allows a neuron to be specifically lesioned in a semi-intact preparation during intracellular recording. Lesioning was achieved by dye-sensitized photoinactivation with a laser epi-illumination stereomicroscope.