Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins

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Science  17 May 1985:
Vol. 228, Issue 4701, pp. 810-815
DOI: 10.1126/science.3923623


Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.