Cell-Assisted Growth of a Fastidious Spiroplasma

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Science  15 Nov 1985:
Vol. 230, Issue 4727, pp. 825-827
DOI: 10.1126/science.230.4727.825


The Colorado potato beetle spiroplasma, which is not cultivable in conventional cell-free media, grew in tissue culture media in the presence of several coleopteran and lepidopteran insect cell lines. The cultured organisms attained titers of 1.2 x 109 spiroplasmas per milliliter of culture at the 100th passage and retained infectivity and a high capacity for translational motility at the 15th passage. Cell culture systems may facilitate the isolation of other presently uncultivable microorganisms and may be useful in the study of the role of microbial physiology and behavior in pathogenicity.