Research Articles

A mammalian mitochondrial RNA processing activity contains nucleus-encoded RNA

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Science  06 Mar 1987:
Vol. 235, Issue 4793, pp. 1178-1184
DOI: 10.1126/science.2434997


Ribonuclease mitochondrial RNA processing, a site-specific endoribonuclease involved in primer RNA metabolism in mammalian mitochondria, requires an RNA component for its activity. On the basis of copurification and selective inactivation with complementary oligonucleotides, a 135-nucleotide RNA species, not encoded in the mitochondrial genome, is identified as the RNA moiety of the endoribonuclease. This finding implies transport of a nucleus-encoded RNA, essential for organelle DNA replication, to the mitochondrial matrix.

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