Estrogen-dependent in vitro transcription from the vitellogenin promoter in liver nuclear extracts

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Science  04 Mar 1988:
Vol. 239, Issue 4844, pp. 1137-1139
DOI: 10.1126/science.2830672


One approach to analyzing the molecular mechanisms of gene expression in vivo is to reconstitute these events in cell-free systems in vitro. Although there is some evidence for tissue-specific transcription in vitro, transcriptionally active extracts that mimic a steroid hormone-dependent enhancement of transcription have not been described. In the study reported here, nuclear extracts of liver from the frog Xenopus laevis were capable of estrogen-dependent induction of a homologous vitellogenin promoter that contained the estrogen-responsive element.