Large microtubule-associated protein of T. brucei has tandemly repeated, near-identical sequences

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Science  22 Jul 1988:
Vol. 241, Issue 4864, pp. 459-462
DOI: 10.1126/science.3393912


The parasitic protozoon Trypanosoma brucei contains a highly organized membrane skeleton, consisting of a dense array of parallel, singlet microtubules that are laterally interconnected and that are also in tight contact with the overlying cell membrane. A high molecular weight, heat-stable protein from this membrane skeleton was isolated that is localized along the microtubules. Protease digestion experiments and sequencing of a cloned gene segment showed that most of the protein is built up by more than 50 nearly identical tandem repeats with a periodicity of 38 amino acids.

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