Antisense RNA-induced reduction in murine TIMP levels confers oncogenicity on Swiss 3T3 cells

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Science  17 Feb 1989:
Vol. 243, Issue 4893, pp. 947-950
DOI: 10.1126/science.2465572


Mouse 3T3 cell lines capable of constitutively synthesizing an RNA complementary to the messenger RNA encoding TIMP, tissue inhibitor of metalloproteinases, were constructed by transfection with appropriate plasmid constructs. Many of the lines were down-modulated for TIMP messenger RNA levels and secreted less TIMP into the culture medium. In comparison to noninvasive, nontumorigenic controls, these cells not only were invasive in a human amnion invasion assay, but also were tumorigenic and metastatic in athymic mice. These results indicate that TIMP suppresses oncogenicity, at least in immortal murine 3T3 cells.

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