Specific recognition of cruciform DNA by nuclear protein HMG1

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Science  24 Feb 1989:
Vol. 243, Issue 4894, pp. 1056-1059
DOI: 10.1126/science.2922595


Cruciform DNA, a non-double helix form of DNA, can be generated as an intermediate in genetic recombination as well as from palindromic sequences under the effect of supercoiling. Eukaryotic cells are equipped with a DNA-binding protein that selectively recognizes cruciform DNA. Biochemical and immunological data showed that this protein is HMG1, an evolutionarily conserved, essential, and abundant component of the nucleus. The interaction with a ubiquitous protein points to a critical role for cruciform DNA conformations.

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