Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase

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Science  10 Mar 1989:
Vol. 243, Issue 4896, pp. 1363-1366
DOI: 10.1126/science.2646717


An analysis of the aminoacylation kinetics of unmodified yeast tRNAPhe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.

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