Mutations in a protein kinase C homolog confer phorbol ester resistance on Caenorhabditis elegans

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Science  31 Mar 1989:
Vol. 243, Issue 4899, pp. 1713-1716
DOI: 10.1126/science.2538925


The tpa-1 gene mediates the action of tumor-promoting phorbol esters in the nematode Caenorhabditis elegans. A genomic fragment that constitutes a portion of the tpa-1 gene was cloned by Tc1 transposon tagging and was used as a probe to screen a nematode complementary DNA library. One of the isolated complementary DNA clones had a nucleotide sequence that predicts a polypeptide of 526 amino acids. The predicted amino acid sequence revealed that the predicted tpa-1 protein sequence is highly similar to protein kinase C molecules from various animals, including man.

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