Altering the genome by homologous recombination

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Science  16 Jun 1989:
Vol. 244, Issue 4910, pp. 1288-1292
DOI: 10.1126/science.2660260


Homologous recombination between DNA sequences residing in the chromosome and newly introduced, cloned DNA sequences (gene targeting) allows the transfer of any modification of the cloned gene into the genome of a living cell. This article discusses the current status of gene targeting with particular emphasis on germ line modification of the mouse genome, and describes the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred.

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